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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in several tumors, which leads to chromosome instability, and so might contribute to tumorigenesis. This protein was discovered to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a vital job in advertising cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have already been described. Two more splice variants have been reported but detailed nucleotide sequence facts is just not however available.
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Blocking peptides are peptides that bind specially towards the goal antibody and block antibody binding. These peptide normally consists of the epitope acknowledged through the antibody.
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This guideline outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This guide outlines further feasible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is precise.
Concentrate on Information Cyclin E belongs to your very conserved cyclin loved ones, whose members are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show unique expression and degradation styles which lead website for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress through S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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